FIG. 8.

Radiation induction of mRNA by RT-PCR for acute phase, latent period and late fibrotic period transcript genes. C57BL/6NHsd mice (n = 30) were irradiated with 20 Gy to the pulmonary cavity. The mice were sacrificed on various days after irradiation and then the lungs were removed, immediately frozen on dry ice, and mRNA was extracted and real time RT-PCR was performed using primers specific for promoters associated with: Panel A: Pulmonary early response genes, NF-κB (NFK-β) (white), NRF2 (purple), SP-1 (red), AP-1 (green); Panel B: endothelial cell specific markers, vWF (white), VEGF (black), FGF1 (red), CCL13 (green), CTGF (blue), IL-6 (purple); or Panel C: genes associated with development of pulmonary fibrosis, MnSOD (white), TGF-β (black), TNF-α (red), Lysl Ox (blue), IGFbp7 (purple), TLR4 (green).