FIG. 9.

MMS350 decreases radiation-induced transcripts of genes associated with pulmonary fibrosis (organizing alveolitis). C57BL/6/NHsd female mice (n =30) were irradiated with 20 Gy to the thoracic cavity with a subset of mice placed on MMS350 in the drinking water on day 100 after irradiation. The mice were sacrificed on day 0, 7, 28, 150 and 200 after irradiation, then the lungs perfused with PBS, removed and frozen on dry ice. RNA was extracted and RT-PCR was performed using primers specific for promoters associated with: Panel A: Pulmonary early response genes, NF-κB (NFK-β), Nrf2, SP-1, AP-1; panel B: endothelial cell specific markers, vWF, VEGF, FGF1, CTGF; or panel C: genes associated with development of pulmonary fibrosis, MnSOD, TGF-β, IGFbp7, TLR4. Significant differences (P < 0.05) in gene expression between irradiated control mice and MMS350 treated mice are indicated by the (*) symbol.