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. 2014 Jan 17;4:3754. doi: 10.1038/srep03754

Figure 3. Akt expression and phosphorylation in huApoE TR mice.

Figure 3

(A) Immunoblotting of total Akt, phosphoryalted Akt (S473) and phosphorylated Akt (T308) in huApoE3 and huApoE4 TR mice at 32 and 72 weeks of age. β-actin was immunoblotted to ensure similar gel loading of the starting material in each sample. The blot is a representative of three independent experiments. Blot images were cropped for comparison. Densitometry analysis of (B) total Akt level relative to β-actin level, (C) phosphorylated Akt(S437) and (D) phosphorylated Akt(T308) level relative to total Akt level, in 32 and 72 weeks old E3 (white bar) and E4 (grey bar) mice was performed using the NIH ImageJ software. Each value represents the mean ± SEM for individual mouse brain sample (n = 3 at each time point for each mouse line). Lower Akt phosphorylation (S473) was detected in 72 weeks E4 TR mice as compared to E3 TR mice at similar age. However, reduced Akt phosphorylation at T308 can be observed in E4 TR mice at 32 and 72 weeks of age as compared to E3 TR mice of similar age. (*p < 0.04; ** p < 0.01, using Student's t-test).