Figure 2. Effect of periplasmic reductases on selenite reduction by S. oneidensis MR-1.

(A) Selenite reduction kinetics of the wild-type strain and fumarate reductase mutant (ΔfccA), nitrate reductase mutant (ΔnapA), nitrite reductase mutant (ΔnrfA). The reduction ability is expressed as the total quantities of reduced selenite in μmol. Error bars indicate the standard error of triplicate cultures; and (B) Selenite reduction rates of the wild-type, ΔfccA, ΔnapA, and ΔnrfA. The linear portion of the kinetics curves (in the initial 12 h) in Fig. 2A was used to calculate the rates. The Adj. R² of each fitting were 0.9980, 0.8624, 0.9567, 0.9906. Error bars indicate the standard error of triplicate cultures.