Fig. 5. Poliovirus infection of WisL cells.

Human lung fibroblast WisL cells were infected with poliovirus and cytoplasmic lysates were collected from 0 – 12 hours post-infection, as indicated at the top of the gel image, and analyzed by Western blot. (A) Western blot analysis with an antibody specific for PCBP2, with the cleaved or full-length forms of the protein indicated to the right of the gel image. (B) Western blot analysis with poliovirus 3CD specific antibodies to verify a productive viral infection. 3CD precursor protein and 3D or 3D′ products are indicated to the right of the gel image. Lane 6 displays extract from mock-infected cells as a control. Molecular weight markers are indicated to the left of the gel image.