Figure 3.

Analysis of the potential of pmGENIE-3 to perform RNAi-mediated knockdown of gene expression in human cells. Human cell lines (HEK293 and MCF-7) were transfected with pmGENIE-3 vectors expressing short hairpin RNA targeting telomerase reverse transcriptase. Following Puromycin selection, the relative expression of telomerase reverse transcriptase was assessed using real- time reverse transcription polymerase chain reaction. Expression values, normalized to GAPDH are shown relative to corresponding control cells (transfected with Trt0). All analyses were performed in triplicate; standard error bars are shown.