Figure 5.

Analysis of the physiological effect of pmGENIE-3-mediated gene knockdown in human cells. Following puromycin selection, telomere length analysis was performed on the same cells that were transfected with the telomerase reverse transcriptase targeting pmGENIE-3 in Figure 3. (a) Sample blots of southern analysis of TRF length for HEK293 and MCF-7. C, no treatment control; 0, pmGENIE-3-Trt0; 1, pmGENIE-3-Trt1; 2, pmGENIE-3-Trt2. (b) Quantitative analysis of mean TRF length for all samples. All analyses were performed in triplicate; standard error bars are shown. P value represents Student's t test.