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Cultured NPCs express CEACAM1a. (A) PCR amplification revealed that cultured C57BL/6 NPCs express transcripts specific for the JHMV receptor CEACAM1a. A 250 bp amplicon, specific for CEACAM1a, was amplified from cDNA generated from total RNA extracted from cultured NPCs; controls included water and splenocytes isolated from non-infected mice. (B) Representative dot plots showing CEACAM1a expression on the cell surface of C57BL/6 splenocytes (control) and cultured NPCs.
