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Virus-specific CD8+ T cells target S510–518 pulsed NPCs for lysis. CTLs were harvested from mice immunized with the DM variant of JHMV and co-cultured at varying effector:target ratios with S510–518 pulsed, IFN-γ-treated NPCs for 4 h, and lactate dehydrogenase released into the supernatant was subsequently measured. Non-IFN-γ-treated RMA/S cells pulsed with 50 μM S510–518 were used as a positive lysis control.
