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. Author manuscript; available in PMC: 2014 Dec 23.
Published in final edited form as: Biochemistry. 2013 Nov 26;52(51):9375–9384. doi: 10.1021/bi401077f

FIGURE 2.

FIGURE 2

Characterization of sulfonamide Lpd inhibitors. (A) Sulfonamides SL932 (circles), SL809 (triangles), SL827 (squares) and SL917 (diamonds) potently inhibit pure recombinant mLpd (closed symbols) but not hLpd (open symbols). Sulfonamide SL827 is competitive with lipoamide (B) substrate and noncompetitive with NADH (C) substrate. DTNB assay used variable lipoamide (in (B), 0.1 to 5 mM) and NADH-lipoamide fluorimetric assay used variable NADH (in (C), 3.33 to 100 μM). Both assays were run with variable SL827 concentrations as shown. Data were fitted in Prism to a nonlinear regression function for competitive or noncompetitive inhibition. Double reciprocal Lineweaver-Burk plots are shown.