Skip to main content
. 2014 Jan 20;20(3):391–402. doi: 10.1089/ars.2012.4634

FIG. 4.

FIG. 4.

siRNA knockdown of Nrf2 in A549 cells blocks induction of Prdx6 expression by KGF. Upper panel, targeted siRNA knockdown reduces Nrf2 in A549 cells. A549 cells were transfected with 20 or 40 pmoles of non-targeting siRNA (Ntg) or siRNA directed against Nrf2 (Nrf2) and cultured for 72 h. β-actin was used as a loading control. The gel shown was edited to excise irrelevant lanes. The vertical gray line marks the location of the excised portion. Lower panel, A549 cells were transfected with the pSEAP2-Basic plasmid (Basic vector) or the same plasmid with the full-length PRDX6 promoter (Prdx6FL) and, where indicated, siRNA; Ntg as a negative control or siRNA targeted against Nrf2. Assays were performed 24 h after treatment. Control; cells not treated with KGF. KGF; cells treated with 20 ng/ml KGF. Values shown are mean±SE (n=3) with untreated pSEAP2-Basic vector set to 1. *p<0.05 compared with vehicle control, †p<0.05 compared with pSEAP2-Basic vector.