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. Author manuscript; available in PMC: 2015 Feb 5.
Published in final edited form as: Enzyme Microb Technol. 2013 Nov 8;55:133–139. doi: 10.1016/j.enzmictec.2013.10.010

Table 2.

Summary of results of biomass and fatty acid yield. The temperature experiments were carried out with 0.4% glycerol and 1 mM IPTG. The glycerol experiment was carried out at 22°C and with 1 mM IPTG. The IPTG experiment was carried out at 22°C and with 0.4% glycerol. Thus, the 22°C data, the 0.4% Glycerol data and the 1 mM IPTG data are exactly the same data.

Total biomass production (g cells/ L Culture) Total mg Fatty Acid/ L Culture
Experimental Condition pET200/D/lacZ DH1-DH2-UMA pET200/D/lacZ DH1-DH2-UMA
16 °C 0.43 ± 0.08 0.37 ± 0.16 59.65 ± 8.88 227.58 ± 33.39
22 °C 0.57 ± 0.06 0.53 ± 0.06 33.18 ± 5.87 115.33 ± 19.44
30 °C 0.66 ± 0.24 0.84 ±0.23 15.00 ± 2.21 22.57 ± 3.50
No Glycerol 0.29 ± 0.01 0.23 ± 0.01 16.48 ± 2.54 80.31 ± 11.75
0.4 % Glycerol 0.57 ±0.06 0.53 ± 0.06 33.18 ± 5.87 115.33 ± 19.44
No IPTG 1.00 ± 0.08 1.03 ± 0.17 117.77 ± 7.32 182.55 ± 16.23.
1 mM IPTG 0.57 ± 0.06 0.53 ± 0.06 33.18 ± 5.87 115.33 ± 19.44