Table 1.
DHM Performance: Z'-Factor Calculation for the Optical Label-Free Modalities Compared
| DHM | OPL from DIC | TIE from BF | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| HeLa | H9c2 | HeLa | H9c2 | HeLa | H9c2 | ||||||||
| Z'-factor for: | Doxo | Chloro | Doxo | Chloro | Doxo | Chloro | Doxo | Chloro | Doxo | Chloro | Doxo | Chloro | |
| cell count | 0.00 | -0.33 | -1.92 | -3.76 | -0.59 | -3.29 | -2.47 | -3.11 | -0.52 | -6.82 | -0.34 | -10.6 | |
| confluency | 0.37 | -0.02 | -0.75 | -32.3 | -0.48 | -23.0 | -4.86 | -5.78 | -0.19 | -4.31 | -2.06 | -0.89 | |
| DHM specific | average OPD | 0.53 | 0.37 | 0.13 | 0.45 | -1.81 | -1.28 | -0.41 | 0.01 | -0.11 | 0.29 | -0.88 | -0.05 |
| CPA analysis | 0.88 | 0.02 | 0.46 | 0.76 | 0.61 | -2.04 | -8.93 | -10.3 | 0.72 | -0.74 | 0.40 | 0.75 | |
Endpoint (24 h) calculated Z -factor for the round (doxorubicin, doxo ) and vesicle (chloroquine, chloro ) phenotypes in the HeLa and H9c2 cell lines for DHM, OPL restored from DIC and TIE (obtained from 3 BF images, described in the Imaging mode comparison section below). Z -factor above 0 are highlighted in green and below 0 in red.
Cell count is per field of view (0.31 mm2). Confluency is the ratio of the cell surface per field area. Average OPD is the mean optical path length difference per surface area.
CellProfiler Analyst (CPA) analysis is the percentage of cell expressing the round (for doxorubicin-treated cells) or vesicles (for chloroquine-treated cells) phenotypes. DHM specific denotes analysis that can only be conducted with a DHM instrument. n = 12-16 wells per conditions.
The values used to calculate this table are provided in the supplementary data.