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. 2014 Jan 20;20(3):432–442. doi: 10.1089/ars.2013.5368

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Copyright 2014, Mary Ann Liebert, Inc.

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FIG. 3. — The Becn1^+/− mice are autophagy deficient and susceptible to CLP. The deficiency of Becn1 was validated in Becn1^+/+ or Becn1^+/− BMDMs via western blot analysis. *p<0.05 (A) The effect of CO on the autophagic function of isolated Becn1^+/+ or Becn1^+/− BMDMs was determined by assessing GFP-LC3B puncta formation (B) in transfected cells by confocal microscopy. BMDM, bone marrow-derived macrophage; GFP, green fluorescence protein. To see this illustration in color, the reader is referred to the web version of this article at www.liebertpub.com/ars