FIG. 4.

CO significantly rescues Becn1^+/+ mice from CLP but not Becn1^+/− mice. (A) Becn1^+/+ or Becn1^+/− mice were subjected to CO that was pre-administrated for 24 h before or continuously after CLP surgery. Rates of survival were determined. p<0.05, log-rank test. (B) After CLP, the plasma levels of inflammatory cytokines in plasma, IL-1β, and IL-18 were measured by ELISA. (C) For determination of CFU, blood and organ homogenates (lung and liver) were serially diluted and then plated on LB plates. The LB plates were incubated for 24 h at 37°C. Statistical significance was determined by nonparametric Mann–Whitney U analysis. *p<0.05.