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. Author manuscript; available in PMC: 2014 Jan 17.
Published in final edited form as: Regul Pept. 2013 Mar 13;184:40–46. doi: 10.1016/j.regpep.2013.03.024

Fig. 5.

Fig. 5

Blocking ALK5 abolishes TGF-β-induced Smad2 and Smad3 activation and PTHrP protein expression. Hep3B cells were treated with ALK5 inhibitor (3 μM) for 45 m followed by treatment with TGF-β1 (40 pmol/L) or vehicle control (0.1% BSA and 4 mM HCl). Cell lysates were harvested at 30 m for Western blotting using antibodies against phospho-Smad2 and -Smad3, Smad2 and Smad3. Cell lysates were harvested at 48 h for Western blotting using antibody against PTHrP. β-actin served as a protein loading control.