Figure 1. Treg Accumulation at the Site of Injury.
(A–C)Cytofluorometric analysesofhindlimb muscle infiltratesafter Ctx injury. (A) Top: Ly6c expression by CD11b+ cells. Middle and bottom: Foxp3 expression by CD4+ cells. Numbers depict % of gated cells. Representative of three experiments. (B and C) Summary data on fraction and number of cells. Mean ± SD (n ≥ 4).
(D) Immunofluorescence microscopy of muscle sections 7 days after injury. The same section without (top) or with (bottom) DAPI. Arrows show Tregs in close contact with regenerating myofibers. Original magnification ×400.
(E) As in (B), but after cryoinjury.
See also Figure S1.