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Conversion of OxIAA to OxIAA-Glc by UGT74D1. (A) Enzymatic reaction catalyzed by UGT74D1. (B) The MS/MS chromatogram for authentic OxIAA-Glc and metabolites of yeast expressing UGT74D1 in the culture medium with OxIAA (Yeast-UGT74D1 + OxIAA) or without OxIAA (Yeast-UGT74D1 - OxIAA). (C) The MS/MS chromatogram for the reaction products of GST–UGT74D1 and boiled GST–UGT74D1.
