Figure 2. CDC37 Is a Universal Kinase-Specific Cochaperone.

(A–C) HSP90 interaction profiles were compared with CDC37 interaction profiles for kinases (A), transcription factors (B), and E3 ligases (C). Plots show mode-normalized, log₂-transformed luminescence values.

(D) 293T cells were infected with a lentivirus expressing an shRNA construct targeting Cdc37 or GFP.

(E) CDC37 is required for kinase::HSP90 interactions. Client proteins were transfected in duplicate into Renilla-HSP90 expressing cells infected with either Cdc37 or GFP shRNA. Forty-eight hours after transfection, interaction with each client was measured with LUMIER. Boxes indicate 25th and 75th percentiles and whiskers 10th and 90th percentiles. p value was calculated with Wilcoxon rank sum test. (F) 3 × FLAG-tagged client proteins were transfected into 293T cells infected with either Cdc37 or GFP shRNA. Forty-eight hours later, clients were immunoprecipitated with an anti-FLAG antibody. Coimmunoprecipitation with endogenous HSP90 was assayed with a monoclonal HSP90 antibody.

See also Figure S1.