Table 1.

Mtb-specific single cytokine T cell responses

Tissue	Time (wks)	PPD					Ag85b
Tissue	Time (wks)	IL-2^a	IFN-γ	IL-17A	TNF-α	Animal No. (%)^b	IL-2	IFN-γ	IL-17A	TNF-α	Animal No. (%)
PBMC
CD4⁺T	3	0.028–0.844	0.014^c	0.038–0.050	0.016	6/6 (100)	NT^d	NT	NT	NT
	6	0.014–0.022	0.013	NS	0.021	6/7 (86)	0.168–16.09	NS^e	NS	NS	2/2 (100)
	8	0.012–0.084	0.010–0.012	0.039	0.012–0.013	6/8 (75)	0.158–0.239	0.023–0.051	0.037–0.046	1.440–2.220	5/5 (100)
	16–18	0.017–0.130	NS	0.061–0.148	0.013	3/8 (38)	0.148–0.373	NS	0.038–0.191	NS	5/8 (63)
CD8⁺T	3	0.238–0.911	0.033–0.296	NS	0.047–0.307	6/6 (100)	NT	NT	NT	NT
	6	NS	0.038–0.139	NS	0.041–0.204	4/7 (57)	0.163–15.19	0.493	NS	NS	2/2 (100)
	8	NS	0.013–0.066	NS	0.048–0.053	6/8 (75)	0.130–0.386	0.121–0.603	0.046–0.137	0.399–1.146	5/5 (100)
	16–18	NS	0.025–0.074	0.141	0.053–0.123	5/8 (63)	0.152–0.212	0.043–0.208	0.093	0.148	3/8 (38)
Tonsil
CD4⁺T	16–18	0.011–0.033	NS	0.158	0.013	3/8 (38)	NT	NT	NT	NT
CD8⁺T	16–18	0.034–0.131	NS	NS	0.088	3/8 (38)	NT	NT	NT	NT
Subm. LN
CD4⁺T	16–18	0.085–0.968	NS	0.066–0.081	4.258	4/8 (50)	0.041	0.011	NS	0.022	3/7 (43)
CD8⁺T	16–18	0.440–0.812	NS	0.136	0.040–0.677	4/8 (50)	NS	NS	NS	NS	0/7 (0)
Ileum
CD4⁺T	16–18	0.043–0.077	0.039–0.044	0.275–0.800	0.057–0.166	4/6 (67)	0.023–0.062	NS	0.597	0.037–0.633	6/7 (86)
CD8⁺T	16–18	0.294	0.171	0.305	0.080–1.068	4/6 (67)	0.014–0.097	0.020–0.095	0.239–0.298	0.454–1.328	6/7 (86)
Colon
CD4⁺T	16–18	0.068–0.363	0.020–0.450	0.182–0.320	0.055–0.166	6/8 (75)	0.066–0.591	0.019–0.600	NS	0.025–0.156	7/8 (88)
CD8⁺T	16–18	0.535	1.360	NS	0.055–0.212	4/8 (50)	0.187–0.525	0.023–1.550	0.204–0.360	0.180–0.259	7/8 (88)
Mes. LN
CD4⁺T	16–18	NS	NS	0.048	0.011–0.166	4/8 (50)	NS	0.067	NS	NS	1/8 (13)
CD8⁺T	16–18	NS	NS	0.018	0.024–0.097	6/8 (75)	0.021–0.029	0.033	NS	0.125	7/8 (88)
Ax. LN
CD4⁺T	16–18	0.040	NS	NS	0.012	2/8 (25)	0.025	NS	NS	NS	1/8 (13)
CD8⁺T	16–18	NS	0.049	NS	0.068–0.138	2/8 (25)	NS	NS	0.014	0.036–0.080	^{5/8 (63)}

Shown are the ranges of the percentage of cytokine positive CD4 or CD8 T cells.

Only a single animal had a positive response

NT= not tested

NS= not significant; responses were not different from media only controls

Depending on cell numbers, not all animals were tested at all time points for antigen-specific responses. Shown are the number of responding animals / total animals tested. In parentheses we report the the percentages of animals with positive responses.