Skip to main content
. 2013 Feb 8;2:36. [Version 1] doi: 10.12688/f1000research.2-36.v1

Figure 2. CAPs modulate proinflammatory cytokine activity in vitro in the absence of bacteria.

Figure 2.

CF IB3-1 cells were cultured in LHC-8 media with supplements, followed by treatment with PBS (black bars), LL-37 (25µM, light grey bars) or WLBU-2 (25µM, dark grey bars) and measurement of the levels of the proinflammatory cytokines IL-1β and TNF-α by quantitative RT-PCR at time points varying from 0–60min. Data represent fold-change in relative transcript levels of proinflammatory cytokines obtained 30min after peptide exposure; both IL-1β and TNF-α showed significant increases in relative transcript levels that decreased by 60min (see raw data file). CAPs, as effector molecules of the innate immune response, may exert an initial protective effect at the level of the epithelial surface against a potential pathogenic or inflammatory challenge.