Fig. 1.

Coomassie-stained gel: Imidazol gradient elution using a NaPO4-buffer system (pH 8.0). 1: standard, 2: unpurified supernatant, 3: flow through, 4: elution, using a NaPO4 buffersystem (pH 8.0) with 10 mM Imidazol, 5: ... with 20 mM imidazol 6: ... with 40 mM imidazol, 7: ... with 100 mM imidazol, 8: ... second elution with 100 mM imidazol (in a 1:10 dilution)