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DESI analysis of 0.5 μg polar lipid extract deposited on the Teflon slides using pure water or methanol as the spray solvent. (a) Analysis with DESI performed close to the MS inlet, gas flow rate of 1.3L/min, solvent flow rate of 3μL/min. (b) Analysis with 4m probe, gas flow rate at 4.3L/min, solvent flow rate at 8μL/min. (c) Signal intensity of plasma-PE (38:6) (m/z=747.52) recorded with probes of different tube lengths from 0.1 to 4.0m.
