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. Author manuscript; available in PMC: 2014 Jun 1.
Published in final edited form as: Synapse. 2013 Mar 5;67(6):265–279. doi: 10.1002/syn.21637

Table 1. Combination of Treatments and Number of mice used for electron microscopic analysis.

Unilateral Acb Injections
rAAV-Cre NR1-KO or rAAV-GFP
Injection
Location
Drug treatment Animal Number
rAAV-GFP Acb Core Saline 3
rAAV-Cre Acb Core Saline 3
rAAV-GFP Acb Core Apomorphine 3
rAAV-Cre Acb Core Apomorphine 3
rAAV-GFP Acb Shell Saline 3
rAAV-Cre Acb Shell Saline 3
rAAV-GFP Acb Shell Apomorphine 3
rAAV-Cre Acb Shell Apomorphine 4

All mice were allowed to recover for 14 days after the viral injections, and then were given s.c injection of saline or apomorphine ( 5mg/kg) five minutes before the AS and PPI testing and 1 hr prior to being anesthetized and having their brain tissue fixed by cardiac perfusion for electron microscopic immunolabeling.