Table 1. Combination of Treatments and Number of mice used for electron microscopic analysis.
| Unilateral Acb Injections rAAV-Cre NR1-KO or rAAV-GFP |
Injection Location |
Drug treatment | Animal Number |
|---|---|---|---|
| rAAV-GFP | Acb Core | Saline | 3 |
| rAAV-Cre | Acb Core | Saline | 3 |
| rAAV-GFP | Acb Core | Apomorphine | 3 |
| rAAV-Cre | Acb Core | Apomorphine | 3 |
| rAAV-GFP | Acb Shell | Saline | 3 |
| rAAV-Cre | Acb Shell | Saline | 3 |
| rAAV-GFP | Acb Shell | Apomorphine | 3 |
| rAAV-Cre | Acb Shell | Apomorphine | 4 |
All mice were allowed to recover for 14 days after the viral injections, and then were given s.c injection of saline or apomorphine ( 5mg/kg) five minutes before the AS and PPI testing and 1 hr prior to being anesthetized and having their brain tissue fixed by cardiac perfusion for electron microscopic immunolabeling.