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. 2013 Dec 3;39(2):125–132. doi: 10.1093/chemse/bjt061

Figure 2.

Figure 2

Behavioral responses of tubulin-GAL4/UAS-Obp28a and tubulin-GAL4/UAS-Obp56h RNAi lines to bitter tastants. (A) Consumption of 9 bitter tastants by tubulin-GAL4/UAS-Obp28a RNAi and tubulin-GAL4/UAS-Obp56h RNAi lines represented as solution intake in millimeters summed over three capillaries. Asterisks denote significant changes in consumption of bitter tastants between females, shown in black bars; males, shown in grey bars, and corresponding consumption of the progenitor control line, shown in open bars, as determined by Tukey’s test (*P < 0.05; **P < 0.005; ***P < 0.0001). (B) Changes in mRNA levels in the probosces of tubulin-GAL4/UAS-Obp28a and tubulin-GAL4/UAS-Obp56h RNAi flies, shown as a percentage of the expression level in the control line (dotted line). Asterisks denote significant changes determined by two tailed Student t tests (**P < 0.005; ***P < 0.0001). (C) Proboscis extension response (PER). Tubulin-GAL4/UAS-Obp28a RNAi flies, represented by black and grey bars for females and males, respectively, and tubulin-GAL4/progenitor control flies (open bars) were stimulated with a 2µl droplet of 0.8mM quinine for up to 5 s. Full proboscis extension in response to the stimulus was counted as a positive response. Asterisks denote significant changes determined by two tailed Student’s t tests (**P < 0.001; ***P < 0.0001).