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. 2014 Jan 17;9(1):e79575. doi: 10.1371/journal.pone.0079575

Figure 5. Detection of specific CD4+ and CD8+ Tcell responses.

Figure 5

The Lymphocytes from lymph nodes and spleen were isolated at 3, 6 and 9 days post-immunization. Lymphocytes were recovered and stained with cell surface markers CD3, CD4 and CD8. The stained cells were analyzed by flow cytometry (a,b). A, Representative flow cytometric plots of lymphocytes in lymph nodes and spleen at day 9 post immunization. B, All date are from n = 3 mice in each group and presented as mean values±standard errors(SE). Asterisks indicate significant differences between the experimental groups: *, p<0.05.