Table 1. Effect of recombinant TCTP in the progression of cell cycle from B cells.
| Time (h) | Ap | G1 | S | G2M | Incorporation | |
| 0 | HsTCTP | 0.0±0.0 | 81.7±3.5 | 8.5±1.1 | 9.7±2.4 | 3.7±0.3 |
| PfTCTP | 0.0±0.0 | 71.3±5.3 | 10.9±3.3 | 17.8±2.1 | 1.7±0.1 | |
| 24 | HsTCTP | 34.7±2.4 | 53.5±3.4 | 8.4±1.2 | 3.4±0.2 | 22.5±2.5 |
| PfTCTP | 43.3±3.4 | 48.8±1.2 | 6.0±1.8 | 2.0±0.4 | 33.0±1.2 | |
| 48 | HsTCTP | 10.8±3.3 | 43.5±4.8 | 26.1±1.5 | 19.6±0.1 | 33.9±1.0 |
| PfTCTP | 35.9±4.0 | 37.2±3.7 | 20.7±1.6 | 6.3±1.9 | 43.6±0.8 | |
| 72 | HsTCTP | 14.1±1.5 | 33.8±1.5 | 29.8±1.3 | 22.3±1.3 | 38.1±3.0 |
| PfTCTP | 43.6±0.5 | 32.8±0.2 | 14.0±0.4 | 9.6±0.2 | 52.4±0.9 | |
| 96 | HsTCTP | 26.0±2.3 | 46.5±2.7 | 13.9±0.6 | 13.5±1.1 | 39.0±0.7 |
| PfTCTP | 41.4±3.4 | 34.6±0.3 | 20.1±3.7 | 3.8±0.1 | 72.4±2.1* | |
| 120 | HsTCTP | 50.8±4.3 | 37.1±3.3 | 8.3±0.8 | 3.8±0.2 | 54.9±0.3 |
| PfTCTP | 61.3±6.9 | 19.3±2.2 | 17.0±4.0 | 2.4±0.7 | 96.4±0.9** |
Splenic B cells (2×106cells/well) were plated with RPMI 1640 supplemented with 10% FBS and incubated with 10 µg/mL of recombinant HsTCTP or PfTCTP labeled with Oregon Green 488 (green) fluorochrome for 24–120 h at 37°C and 5% CO2. The percentage of cell cycle distribution and incorporation of fluorescently-labeled of PfTCTP and HsTCTP into splenic B cells was analyzed by flow cytometry (FACS). Results (%) are expressed as mean ± SD of duplicate determinations. One of two similar experiments is shown. Incorporation, percentage of incorporation of fluorescently-labeled protein into B cells.
*, p<0.05 vs 10 µg/mL labeled HsTCTP treated B cells
**, p<0.01 vs 10 µg/mL labeled HsTCTP treated B cells