Figure 5. H9 clonogenicity is inhibited by exogenous bFGF in a dosage dependent manner.

(a). Initial H9-0 ng or H9-0.4 ng/H9-4 ng culture was dissociated into single cells and plated into 0 vs. 0.04 vs. 0.4 vs. 4 ng/ml bFGF media, or 0 vs. 4 ng/ml bFGF media, respectively, at 5×10⁴ cells/well. (b). Representative images of ALP stained cultures as described in (a). (c). Left-hand five columns: Initial H9-0.4 ng culture was dissociated into single cells and plated into 0 vs. 0.04 vs. 0.4 vs. 4 ng/ml bFGF or 4 ng/ml bFGF after 2 days (2D) of culture in 0 ng/ml bFGF media. Right-hand three columns: initial H9-0 ng, H9-0.4 ng and H9-4 ng culture were each plated as single cells into its corresponding culture condition, at 5×10⁴ cells/well. (d). Representative images of ALP stained cultures as described in (c). The average number of colonies from triplicate wells was presented for each culture (n = 1). Error bars represent standard deviation. *: P<0.01; **: P<0.05; ***: P<0.1.