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Expression of HSP90, a house keeping gene, was detected in all samples including mitomycin C treated hMSCs feeder cells (MtC-hMSCs). Expression of Nanog, Oct4 and GDF3 were restricted to all pluripotent cell samples and not expressed in MtC-hMSCs (H1 and H9, n = 3; DF19, n = 2). Expression of all genes was negative in water and RNA control sample lanes (not shown). See also Figure S4.
