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. 2014 Jan 17;9(1):e86684. doi: 10.1371/journal.pone.0086684

Figure 1. Atg3 and Atg5 promote the delivery of IFN-inducible GTPases to T. gondii PVs.

Figure 1

(A) Wildtype (WT), Atg3−/− and Atg5−/− MEFs were treated overnight with 200 U/ml of IFNγ or were left untreated. Protein extracts were analyzed by Western blotting using antibodies reactive to Atg3, Atg5, p62, LC3, Gbp2 and actin. (B–D) WT, Atg3−/− and Atg5−/− MEFs were treated overnight with 200 U/ml of IFNγ prior to infections. Localization of endogenous Irgb10 (B and D) and Gbp2 (C and D) to T. gondii PVs was monitored at 0.5 hpi. Data are representative of three independent experiments (**, p<0.005 relative to wildtype). Error bars represent standard deviations. Representative confocal images of T. gondii-infected MEFs are shown in B and C.