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. 2013 Oct 23;39(3):727–737. doi: 10.1038/npp.2013.258

Figure 4.

Figure 4

Place conditioning with ganaxolone (GAN) in adult C57BL/6J and δ-KO mice. (a–e) Results from experiments with C57BL/6J mice: (a) locomotor activity during each conditioning trial with two doses of GAN (conditioning stimulus (CS)+) and with vehicle (CS−) (vehicle trial data from both dose levels were combined). Conditioning time for each vehicle and active drug trial was 30 min. *P<0.05, compared with vehicle group (ANOVA with Bonferroni test). (b) Locomotor activity in 5-min time-bins during the first conditioning trial. *P<0.05 and ***P<0.001, compared with vehicle group. (c) C57BL/6J mice conditioned with GAN at a dose of 30 mg/kg expressed place aversion at 24 h after the last conditioning session, whereas those conditioned with the lower dose failed to express any preference or aversion. Test trial time after vehicle injection was 30 min. *P<0.05. (d) Locomotor activity during place conditioning test trial. Data for C57BL/6J mice are presented as means±SEM, n=14–25. (e) GAN-induced hypothermia in C57BL/6J mice after treatment with GAN 30 mg/kg, 4,5,6,7-tetrahydroisoxazolol[4,5-c]pyridine-3-ol (THIP) 6 mg/kg or vehicle. *P<0.05. (f–j) Results from experiments with γ-aminobutyric acid type-A (GABAA) receptor δ-subunit-knockout (δ-KO) mice and their WT littermates (δ-WT): (f) locomotor activity during each conditioning trial. GAN (30 mg/kg) conditioning was performed identically as with C57BL/6J mice. (g) Locomotor activity in 5-min time-bins during the first conditioning trial. The inset shows the percentage of total distance moved in the center of the arena during the first conditioning trial, indicating increased activity in the WT mice after GAN. *P<0.05, compared with the corresponding vehicle group (ANOVA with Bonferroni test). (h) WT mice expressed GAN-induced conditioned place aversion (CPA), whereas the KO mice displayed neither preference nor aversion. **P<0.01. (i) Locomotor activity during place-conditioning test trial. (j) GAN-induced hypothermia in δ-KO mice after treatment with GAN 30 mg/kg, THIP 6 mg/kg or vehicle. *P<0.05. Data for δ-WT and δ-KO mice are presented as means±SEM, n=7–11.