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. 2014 Feb;20(2):177–188. doi: 10.1261/rna.042358.113

FIGURE 4.

FIGURE 4.

Ribosome binding of U34 mutants of H. marismortui tRNA2Ile and tRNA1Ile. Template-dependent binding of purified 3H-Ile-tRNA2Ile wild-type (A) and 3H-Ile-tRNA2Ile U34 (B) to ribosomes isolated from H. marismortui. Oligonucleotides used were AUGAUA, AUGAUC, AUGAUG, and AUGAUU. (C) Binding of purified 3H-Ile-tRNA2Ile U34 and 3H-Ile-tRNA1Ile U34 to ribosomes isolated from H. volcanii; the mRNA concentration was 1 mM; the AUGUUU and “no mRNA” controls are also shown in C. Results from three independent experiments are shown.