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Verification of cloning by colony PCR and restriction enzyme digestion. (A) Some colonies showed to be recombinant by revealing a band of about 1300 bp following colony PCR using OpIE2Fr and OpIE2Rv primers. (B) Restriction endonuclease digestion of the prepared plasmid by HindIII and XhoI enzymes confirmed the cloning by releasing a band of about 1100 bp in some clones (1), while the other clones showed to be not recombinant (2). Mw; Molecular weight marker.
