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Dot-blot analysis of the recombinant stable clones of the Sf9 insect cells. Evaluation of the concentrated cell culture medium of the obtained stable cell lines revealed the expression of reteplase by some clones (4, 5 and 8) while the other clones were not capable of its expression. (pc) positive control for anti V5 tag; (nc) concentrated cell culture medium of cells transfected with non-recombinant pMIB/V5-His plasmid as negative control.
