Skip to main content
. Author manuscript; available in PMC: 2014 Jul 7.
Published in final edited form as: Nat Commun. 2014;5:3051. doi: 10.1038/ncomms4051

Figure 7. Effects of an m-calpain specific inhibitor on TEA-mediated SCOP and PTEN degradation and on LTP.

Figure 7

a–f: Hippocampal slices were prepared from adult rats and were treated with TEA (20 mM) for 10 min, and collected at the indicated times. TEA and mCalp-I (200 nM) were applied as shown in a and d. b and e: Representative western blots for SCOP, PTEN and Akt at the indicated times. c and f: Quantitative analysis of the levels of SCOP and PTEN (normalized to the values of Akt). In all cases, results are means ± S.E.M. of 3 experiments. * p < 0.05, as compared to time point 0, one-way ANOVA followed by Bonferroni test. g–h. Effects of mCalp-I on TBS-induced LTP. mCalp-I (200 nM) was applied either before TBS (vertical arrow) (blue circles) or 20 min after TBS (red circles). mCalp-I alone had no effect on baseline responses (blue triangles).

h. Summary of the amplitude of LTP measured within the gray area corresponding to 49–50 min after TBS. * p < 0.01 as compared to TBS alone.