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. Author manuscript; available in PMC: 2014 Jan 18.
Published in final edited form as: Cell Host Microbe. 2012 Sep 20;12(4):509–520. doi: 10.1016/j.chom.2012.08.004

Figure 5.

Figure 5

Gadd45α on DCs is required for in vitro IL-10 production and protection from colitis by OMVs. (A) ELISA analysis of DC-T co-cultures similar to Figure 3(C), but also including Gadd45α-/- DCs. Error bars indicate SEM from quadruplicate samples. Results are representative of 3 independent trials. *p<0.05. ** p<0.01. NS: not significant. (B) qRT-PCR analysis of IL-10 transcript levels from either WT or Gadd45α-/- BMDCs after 16-18hrs incubation with OMVs in vitro. Error bars indicate SEM from triplicate samples. Results are representative of 3 independent trials. ** p<0.01. NS: not significant. (C) Colitis scores from DC recipient animals induced for TNBS colitis. Each symbol represents an individual animal. Results are shown from 2 combined experiments, each performed independently. (D) Colon length from mice, at day 2 following TNBS treatment. Each symbol represents an individual animal. Error bars indicate SEM from 8 animals. Results are shown from 2 combined experiments each performed independently. * p<0.05. *** p<0.001. NS: not significant. (E) Cytokine analysis by qRT-PCR of RNA recovered from whole colon. Each symbol represents an individual animal. Error bars indicate SEM from 8 animals. Results are shown from 2 combined experiments, each performed independently. See also Figure S5.