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Figure 2. Time-dependent increase of chromatin-associated PTEN. HeLa cells were treated with 1 mM H2O2 for various times as indicated. Cells were collected and cytoplasmic and chromatin-bound fractions were obtained. Equal amounts of lysates from each fraction were blotted for phospho-PTEN (epitopes T366/S370 and S380, respectively), total PTEN, phospho-H2AX, and actin.
