Figure 5. PTEN specifically interacts with Rad52. (A) HEK293T cells were transfected with plasmid expressing Flag-tagged PTEN for 48 h. Equal amounts of cell lysates prepared from various transfection samples were immunoprecipitated with the antibody to Flag. Immunoprecipitates, along with lysate inputs, were then blotted for Flag. ATR, Rad50, and Rad52. (B) HeLa Cells were treated 1 mM H₂O₂ for 24 h and then fixed and stained with antibodies against PTEN (green) and Rad52 (red). DNA was stained with DAPI. Representative images are shown. (C) HeLa Cells were treated 1 mM H₂O₂ for 24 h and then fixed and stained with antibodies against PTEN (green) and phospho-H2AX (γH2AX, red). DNA was stained with DAPI. Representative images are shown.