Fig. 3.

NG2 domain-specific fusion proteins exhibit differences in plasmin generation. Individual NG2 domain-Fc fusion proteins were used at 3 nM in a tPA-catalyzed plasmin generation assay without (A) or with (B) chondroitinase pretreatment. The activities observed were normalized over the nonenhanced tPA/plasminogen control to enable presentation as fold-increases. The expression levels and integrity of the recombinant fusion proteins were confirmed by western blot analysis (data not shown). Error bars represent S.E.M.