Fig. 7.

Plasmin-mediated NG2 degradation and neurite outgrowth. Cortical neurons were plated onto nitrocellulose-coated dishes containing (A) NG2; (B) Chondroitinase ABC digested NG2; (C) Plasmin proteolyzed NG2; (D) NG2 treated with both chondroitinase ABC and plasmin or (E) laminin. Cultures were photographed after 5 days of growth. Phase-contrast images of cortical neuronal cells are shown. (F) Quantification of neurite length was performed using Metamorph software. Statistical analysis was performed using paired, two-tailed Student’s t-test. Scale bar denotes 100 μm.