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. Author manuscript; available in PMC: 2015 Jan 1.
Published in final edited form as: Curr Pharm Des. 2014;20(11):1655–1663. doi: 10.2174/13816128113199990538

Figure 3.

Figure 3

Sample MLH1 methylation chromatogram. Panel 1 shows analysis of the negative control leukemia cell line K562, which is known to lack MLH1 methylation, while panel 2 demonstrates a different peak for the colon cancer cell line RKO, which is known to have loss of MLH1 protein expression secondary to MLH1 methylation. Panel 3 is for normal tissue from an endometrial cancer, and panel 4 is for an endometrial carcinoma. Note in panel 4 the presence of 2 peaks, methylated and unmethylated. The presence of the unmethylated peak is likely due to the presence of a minor population of normal stromal cells or inflammatory cells which will not have methylation of MLH1.