(A) Adoptively transferred CD45.1+CD4+ Tregs are present in the spleen, bone 17 marrow (BM), lymph node (LN), blood, lung, and brain at 7 days after MCAO. Plots are representative of four animals. (B) Cell tracker-labeled Tregs were observed in the lung, liver, spleen, and brain 7 days after MCAO. Images are representative of four animals.