Figure 1.

Confirmation of the presence of 465 bp hbx fragment in pcDNA3 vector by 3 methods; A) Colony PCR. Lane 1: 100 bp DNA molecular size marker, Lane 2: PCR product of pcDNA3 plasmid without HBX gene (negative control), Lane 3: PCR product of pcDNA3-HBX vector; B) Restriction endonucleases digestion. Lane 1: 100 bp DNA molecular size marker, Lane 2: pcDNA3- HBX without digestion, Lane 3 and 4: Digestion product of pcDNA3-HBX with EcoRI and Hind III, Lane 5: Digestion product of pcDNA3 (control); C) The direct sequencing result of pcDNA3-HBX