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. 2013 Dec 12;9:251. doi: 10.1186/1746-6148-9-251

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Copyright © 2013 Bracha et al.; licensee BioMed Central Ltd.

This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Phosphorylation of ERK is cell line and treatment dependent. a: Immunoblots of ERK and phosphorylated ERK in normal osteoblasts. CnOb exhibited decreased ERK phosphorylation when treated with increasing concentrations of serotonin (S), ritanserin (R) or when both drugs are combined (SR). b: Immunoblots of ERK and phosphorylated ERK in COS. Treatment with increasing concentrations of serotonin (S), ritanserin (R), or both drugs (SR) resulted in commensurate increases in phosphorylation.