Table 1.
Primer sequences
| Gene | Oligo names | Primer sequences 5′-3′ |
|---|---|---|
| |
CdICE1-B |
ACTGGATCCATGCTACCGGAAAACGACA |
| CdICE1-S |
TCCCCCGGGAATGGCACCATGATAACCT |
|
| |
zh-F |
CGGGTACCATGCTACCGGAAAACGACA |
| zh-R |
TCCCCGCGGTAATGGCACCATGATAACCT |
|
| |
ICE1 |
ACTCATATGATGCTACCGGAAAACGACA |
| |
ICE21 |
ACTCATATGACCCCTACAACCACCCCA |
| |
ICE85 |
ACTCATATGTCACCTTCACAATCACACTCAC |
| |
ICE121 |
ACTCATATGTGTGACCCTAGCTTCATTTCCA |
| |
ICE471 |
ACTGGATCCAATGGCACCATGATAACCT |
|
CdICE1 |
CdICE-F |
CCTACCCAACAAATGGCAACTA |
| CdICE-R |
TCTCTCCACTCTGTCTCAGCGC |
|
|
CBF1 |
cbf1-F |
GTGACGTGTCGCTTTGGAGTTAC |
| cbf1-R |
GTGAAGCAAAGAAGTAGAAAACG |
|
|
CBF2 |
cbf2-F |
TCGAGGGAGATGATGACGTGTCC |
| cbf2-R |
TATTTTGATTTGTTGCTTATGG |
|
|
CBF3 |
cbf3-F |
CGACGGCGATGATGACGACGT |
| cbf3-R |
GCATTTAAGAATAGCCCACAC |
|
|
COR6.6 |
cor6.6-F |
CAGAGACCAACAAGAATGCC |
| cor6.6-R |
CGATATACTCTTTCCCGCCT |
|
|
COR15a |
cor15a-F |
AAAGCAGGAGAGGCTAAGGAT |
| cor15a-R |
CATGAAGAGAGAGGATATGGATCA |
|
|
RD29a |
rd29a-F |
TAGGAAAGTAAAGGCTAGAGCTAAG |
| rd29a-R |
AATCGGAAGACACGACAGG |
|
|
β-tubulin |
tubin-F |
AAGATTCGTCCCACGCG |
| tubin-R |
TCCTTTAGCCCAATTGTTACC |
|
|
U6 |
U6-F |
CTCGCTTCGGCAGCACA |
| U6-R |
AACGCTTCACGAATTTGCGT |
|
|
CSD2 |
CSD2-F |
AACCCTAACAACATGACACACG |
| CSD2-R |
GAACCACAAAGGCTCTTCCAAC |
|
|
CSD1 |
CSD1-F |
AGACCCTGATGACCTCGGAAA |
| CSD1-R |
GCCACACACCAGAAGATACAC |
|
|
miR398 |
miR398-F |
TGTGTTCTCAGGTCACCCCT |
| Uni-miR qPCR Primer |
From TaKaRa |
|
| MIR398a |
miR398a-F |
AGAAGAAGAGAAGAACAACAGGAGGTG |
| miR398a-R |
TTTAGTAAGGTGAAAAAATGGAACAGG |
|
| MIR398b |
miR398b-F |
TAACAAGAAGATATCAATATATCATG |
| miR398b-R |
ACCATTTGGTAAATGAGTAAAAGCCAGCC |
|
| MIR398c |
miR398c-F |
TCGAAACTCAAACTGTAACAGTCC |
| miR398c-R | ATTTGGTAAATGAATAGAAGCCACG |