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. 2013 Nov 27;2:15. doi: 10.1186/2046-2530-2-15

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Copyright © 2013 Lechtreck et al.; licensee BioMed Central Ltd.

This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Microtubule formation during CP assembly. Zygotes obtained by mating pf15 with a WT strain expressing triple HA-tagged α-tubulin were analyzed by immunofluorescence microscopy using anti-α-tubulin (a1–e1), anti-HA (a2–e2), and anti-hydin (a3–e3). Merged images are shown in a4–e4. Arrows in a1–e1: flagella derived from pf15. Filled arrowheads: developing CP as detected with anti-HA and anti-hydin. Open arrowheads: incorporation of HA-tubulin at the distal end of flagella derived from pf15. Bar = 10 μm.