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Non-invasive circadian clock detection with hair follicle cells. Overview from sampling to quantification by realtime-PCR (RT-PCR). Plucking head hair sharply at the roots with non-slip tweezers in the direction of hair growth tends to extract a good deal of hair follicles. Pre-analysis, samples can be stocked in a deep freezer. The standard protocol for RT-PCR is adopted for the analysis. The graph in this diagram is adapted from Akashi et al. (2010).
