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Primers used for pyrosequencing assessments of SLC19A1 methylation
| Step | Name | Sequence |
|---|---|---|
| PCR1 |
RFC1F1 |
AGGGATAAGTATAGTTTGTTTTTGGGGAT |
| PCR1 |
RFC1R |
AATAACCCAAACCCCCCTTCC |
| PCR1 |
RFC1RU |
GGGACACCGCTGATCGTTTAATAACCCAAACCCCCCTTCC |
| PCR2 |
RFC1F2 |
GTGATTAGTAAGGGTTTGTATTAAGGAGTAAG |
| PCR2 |
BioUni |
[Biotin]GGGACACCGCTGATCGTTTA |
| PSQ | RFC1S | TAGTTTTTATTTTAGTAGGGATAG |
