Figure 7. Lensfree simultaneous multicolor fluorescent imaging on a chip.

The performance of the decoding method summarized in Fig. 6 is demonstrated with (a and b) 4 μm green fluorescent beads and (c and d) 10 μm red and green fluorescent beads. (a1, b1, c5 and d5) Raw, lensfree fluorescence signatures of these microbeads are cropped from a large field-of-view of ~2.42 cm². These raw images are decoded to retrieve color and spatial distribution information. (a3, b3, c6, c7, c8, d6, d7, and d8) Decoding results are verified with (a4 and b4 insets) bright-field and (c3, c4, d3, and d4) fluorescent microscope images of the same objects. (a2, b2, c1, and d1) The debayered lensfree images illustrate the limitations of the color sensor for lensfree fluorescent imaging without our demultiplexing method. The arrows are highlighting two regions, where our method effectively resolves the color as well as spatial overlap of lensfree fluorescent signatures.