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. Author manuscript; available in PMC: 2014 Aug 1.
Published in final edited form as: Cancer Immunol Res. 2013 Aug;1:99–111. doi: 10.1158/2326-6066.CIR-13-0047

Figure 1.

Figure 1

TRP1 TN mice were derived from TRP1-specific CD8 T cells. A) C57BL/6x129 F1 agouti male mice were immunized with TRIVAX (palmitoylated TAPDNLGYM 10μg/mouse, anti-CD40 30μg/mouse and poly-IC 15 μg/mouse) intravenously. Splenocytes were harvested 6 or 8 days post-immunization and stained with anti-CD8 and TRP1 tetramer. Gated cells were sorted by FACS and used as nucleus donors for SCNT. B) Success rate of nuclear transfer. C) ES cell lines 6.15 and 6.17 were injected into wild type blastocysts to generate chimeric mice as shown. Germline transmission was achieved by crossing chimeras to C57BL/6 females. D) Peripheral blood mononuclear cells were harvested from germline transmitted 6.15 or 6.17 mice and stained with TRP1 tetramer, anti-CD4 and anti-CD8. The calculated CD4:CD8 ratio is shown below each plot. Note the lower intensity tetramer staining in the 6.15 mouse, representative of more than 100 mice analyzed.